safety pharmacology

List* of tests performed at BioTox by Icare

BioTox by Icare offers safety pharmacology tests in accordance with Good Laboratory Practices.


Central nervous system:

  • Neurobehavioral observation test: Irwin test / ICH S7A
  • Motor coordination: Rotarod test / ICH S7A
  • Pro-convulsant effect / ICH S7A
  • Effect on alertness: interaction with hexobarbital-induced sleep / ICH S7A

Gastrointestinal system: 

  • Study of effects on intestinal transit: anthrax transit time / ICH S7A
  • Effect on gastric emptying and gastric secretions / ICH S7A
  • Ulcerogenic effect / ICH S7A

Analgesia / Coagulation: 

  • Central analgesia: Hot Plate test / ICH S7A
  • Central and peripheral analgesia: Randall-Selitto test / ICH S7A
  • Anti-inflammatory effect: paw oedema volume test / ICH S7A
  • Coagulation time / ICH S7A

Toxicological studies:

  • Cytotoxicity in human cells (fibroblasts, melanocytes, keratinocytes, adipocytes, endothelial cells, etc.)
  • In vitro eye irritation (neutral red/SIRC or diffusion/agarose gel /L929 according to Official Journal)
  • Phototoxicity in Balb/c 3T3 (OECD 432)
  • Skin irritation on reconstructed human epidermis
  • Inflammatory potential on reconstructed human epidermis
  • Acute toxicity in mice (OECD 420)(**)
  • Skin irritation/corrosion in rabbits (OECD 404)(**)
  • Eye irritation/corrosion in rabbits (OECD 405)(**)
  • Sensitisation in guinea pigs (OECD 406)(**)           
  • Pyrogenic effect in rabbits (European Pharmacopoeia)(**)
  • Subacute toxicity in rats (4 weeks) (OECD 407)(**)
  • Subchronic toxicity in rats (12 weeks) (OECD 408)(**)  
  • Carcinogenesis in rats (OECD 451)(**)
  • Reproductive and developmental toxicity (OECD 415, OECD 416, OECD 414, OECD 421)(**)

DNA damage, mutagenicity, genotoxicity:

  • 5 or 6-strain Ames test (Salmonella typhimuriumEscherichia coli) (OECD 471)
  • Testing for chromosomal aberrations in mammalian cells (human lymphocytes, CHO, V79, TK6) (OECD 473)
  • Gene mutation test in mammalian cells (L5178Y, V79, TK6) (OECD 476,OECD 490)
  • In vitro micronucleus test in mammalian cells (CHO, TK6) (OECD 487)
  • COMET test (DNA damage, DNA protection) (OECD 489)
  • Micronucleus test in rats (OECD 474)(**)


Development of alternative tests to animal testing.

*Non-exhaustive list: for more information, click here!

(**)Tests performed in partnership, in accordance with Good Laboratory Practices

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