safety pharmacology
List* of tests performed at BioTox by Icare
BioTox by Icare offers safety pharmacology tests in accordance with Good Laboratory Practices.
Central nervous system:
- Neurobehavioral observation test: Irwin test / ICH S7A
- Motor coordination: Rotarod test / ICH S7A
- Pro-convulsant effect / ICH S7A
- Effect on alertness: interaction with hexobarbital-induced sleep / ICH S7A
Gastrointestinal system:
- Study of effects on intestinal transit: anthrax transit time / ICH S7A
- Effect on gastric emptying and gastric secretions / ICH S7A
- Ulcerogenic effect / ICH S7A
Analgesia / Coagulation:
- Central analgesia: Hot Plate test / ICH S7A
- Central and peripheral analgesia: Randall-Selitto test / ICH S7A
- Anti-inflammatory effect: paw oedema volume test / ICH S7A
- Coagulation time / ICH S7A
Toxicological studies:
- Cytotoxicity in human cells (fibroblasts, melanocytes, keratinocytes, adipocytes, endothelial cells, etc.)
- In vitro eye irritation (neutral red/SIRC or diffusion/agarose gel /L929 according to Official Journal)
- Phototoxicity in Balb/c 3T3 (OECD 432)
- Skin irritation on reconstructed human epidermis
- Inflammatory potential on reconstructed human epidermis
- Acute toxicity in mice (OECD 420)(**)
- Skin irritation/corrosion in rabbits (OECD 404)(**)
- Eye irritation/corrosion in rabbits (OECD 405)(**)
- Sensitisation in guinea pigs (OECD 406)(**)
- Pyrogenic effect in rabbits (European Pharmacopoeia)(**)
- Subacute toxicity in rats (4 weeks) (OECD 407)(**)
- Subchronic toxicity in rats (12 weeks) (OECD 408)(**)
- Carcinogenesis in rats (OECD 451)(**)
- Reproductive and developmental toxicity (OECD 415, OECD 416, OECD 414, OECD 421)(**)
DNA damage, mutagenicity, genotoxicity:
- 5 or 6-strain Ames test (Salmonella typhimurium, Escherichia coli) (OECD 471)
- Testing for chromosomal aberrations in mammalian cells (human lymphocytes, CHO, V79, TK6) (OECD 473)
- Gene mutation test in mammalian cells (L5178Y, V79, TK6) (OECD 476,OECD 490)
- In vitro micronucleus test in mammalian cells (CHO, TK6) (OECD 487)
- COMET test (DNA damage, DNA protection) (OECD 489)
- Micronucleus test in rats (OECD 474)(**)
Development of alternative tests to animal testing.
*Non-exhaustive list: for more information, click here!
(**)Tests performed in partnership, in accordance with Good Laboratory Practices