Toxicology – Pharmaceutical industry

Toxicological studies:

• Cytotoxicity on human cells (fibroblasts, melanocytes, keratinocytes, adipocytes, endothelial cells… )
• Skin irritation on reconstructed human epidermis
• Acute toxicity in mice (OECD 420, 423, 425)(*)
• Irritation / Skin corrosion in rabbits (OECD 404)(*)
• Irritation / Eye corrosion in rabbits (OECD 405)(*)
• Sensitization in guinea pigs & mice (OECD 406)(*)
• Subacute toxicity in rats (4 weeks) (OECD 407)(*)
• Subchronic toxicity in rats (12 weeks) (OECD 408)(*)
• Carcinogenicity in rats (OECD 451)(*)
• Reproductive and developmental toxicity (OECD 415, OECD 416, OECD 414, OECD 421)(*)

DNA damage, mutagenicity, genotoxicity :

• Ames 5- or 6-strain test (Salmonella typhimurium, Escherichia coli) (OECD 471)
• Chromosome aberration test in mammalian cells (human lymphocytes, CHO, V79, TK6) (OECD 473)
• Gene mutation test in mammalian cells (L5178Y, V79, TK6) (OECD 476, OECD 490)
• In vitro micronucleus test on mammalian cells (CHO, TK6) (OECD 487)
• Micronucleus test in rats (OECD 474)

Development of alternative tests to animal experimentation.

(*) tests carried out in partnership in compliance with Good Laboratory Practice

Analgesia / Coagulation:

• Central analgesia: Hot Plate test / ICH S7A
• Central and peripheral analgesia: Randall and Selitto test / ICH S7A
• Anti-inflammatory effect: paw edema volume test / ICH S7A
• Clotting time / ICH S7A