Toxicology – Pharmaceutical industry
In Vitro and In Vivo tests
Central nervous system:
- Neurobehavioral observation test: Irwin test / ICH S7A
- Motor coordination: Rotarod test / ICH S7A
- Pro-convulsant effect / ICH S7A
- Effect on alertness: interaction with hexobarbital-induced sleep / ICH S7A
Gastrointestinal system:
- Study of effects on intestinal transit: charcoal transit time / ICH S7A
- Effect on gastric emptying and gastric secretions / ICH S7A
- Ulcerogenic effect / ICH S7A
Toxicological studies:
- Cytotoxicity on human cells (fibroblasts, melanocytes, keratinocytes, adipocytes, endothelial cells… )
- In vitro eye irritation (neutral red/SIRC or diffusion/agarose gel/L929 according to OJ)
- Phototoxicity on Balb/c 3T3 (OECD 432)
- Skin irritation on reconstructed human epidermis
- Inflammatory potential on reconstructed human epidermis
- Acute toxicity in mice (OECD 420)(**)
- Irritation / Skin corrosion in rabbits (OECD 404)(**)
- Irritation / Eye corrosion in rabbits (OECD 405)(**)
- Sensitization in guinea pigs (OECD 406)(**)
- Pyrogenicity in rabbits (European Pharmacopoeia)(**)
- Subacute toxicity in rats (4 weeks) (OECD 407)(**)
- Subchronic toxicity in rats (12 weeks) (OECD 408)(**)
- Carcinogenicity in rats (OECD 451)(**)
- Reproductive and developmental toxicity (OECD 415, OECD 416, OECD 414, OECD 421)(**)
DNA damage, mutagenicity, genotoxicity :
- Ames 5- or 6-strain test (Salmonella typhimurium, Escherichia coli) (OECD 471)
- Chromosome aberration test in mammalian cells (human lymphocytes, CHO, V79, TK6) (OECD 473)
- Gene mutation test in mammalian cells (L5178Y, V79, TK6) (OECD 476, OECD 490)
- In vitro micronucleus test on mammalian cells (CHO, TK6) (OECD 487)
- COMET test (DNA degradation, DNA protection) (OECD 489)
- Micronucleus test in rats (OECD 474)(**)
Development of alternative tests to animal experimentation.
(**) tests carried out in partnership in compliance with Good Laboratory Practice
Analgesia / Coagulation:
- Central analgesia: Hot Plate test / ICH S7A
- Central and peripheral analgesia: Randall and Selitto test / ICH S7A
- Anti-inflammatory effect: paw edema volume test / ICH S7A
- Clotting time / ICH S7A
In vitro test Chemical products
Eye irritation:
- Reconstituted human corneal epithelium model OECD 492 (EpiOcular)
- Reconstituted human corneal epithelium model OECD 492B (SkinEthic)
- Acute eye irritation OECD 405
Skin irritation / Skin corrosion:
- Reconstructed human epidermis OECD 439
- Reconstructed human epidermis (corrosion) OECD 431
- In vitro membrane test OECD 435
Skin sensitization:
- In chemico skin sensitization (DPRA)* OECD 442C
- Keratinosens test method* OECD 442D
- Human cell line activation test (h-CLAT)* OECD 442E
Mutagenicity & genotoxicity:
- Gene mutation test on mammalian cells using the thymidine kinase gene OECD 490
- Reverse mutation test on bacteria (Ames) OECD 471
- Chromosome aberration test on human or rodent cells OECD 473
- In vitro micronucleus test on mammalian cells (CHO) OECD 487
** Tests carried out in partnership in accordance with Good Laboratory Practice.
For other tests, please contact us.
